TentaGel® PAP - TentaGel® Resin for the Synthesis of PEG Attached Peptides

€ 62,00*

SIZE

90 µm

Capacity

0.18 - 0.25 mmol/g

AMOUNT

Product number
J1002.1G

Product information

TentaGel^® resins are grafted copolymers consisting of a low crosslinked polystyrene matrix on which polyethylene glycol (PEG or POE) is grafted via an ethyl ether group which increases stability towards acid treatment and minimizes PEG-leaching. The graft copolymer shows modified physicochemical properties which are highly dominated by the PEG moiety (and no longer by the polystyrene matrix) and has hydrophobic and hydrophilic properties. These copolymers are pressure stable and can be used in batch processes as well as under continuous flow conditions. The PEG spacer is in the range of MW 3000 Da. TentaGel^® resins have high diffusion rates and excellent swelling in a wide range of solvents from e.g. toluene to water.
In this special TentaGel^® resin the PEG spacer is attached to the polystyrene/DVB matrix by a cleavable linkage. Therefore, the PEGylated compound can easily be cleaved and separated from the insoluble support. This can be achieved with either 10 mL/g of TFA/thioanisole (95:5) for 12 hours at room temperature, or with 10 mL/g of TFA/trimethylsilyl bromide/thioanisole (94:1:5) for 1 hour at room temperature.

Literature

L 19 PEG peptides and Pam peptides

Rapp W., Zhang L., Beck-Sickinger A. G., Dares K., Wiesmüller K.-H., Jung G., Bayer E. Comparative study of antibody titers induced by a peptide epitope conjugated with protein, lipopeptide, polyoxyethylene and polyoxyethylene-polystyrene graft copolymer in Peptides 1990, Proceedings of the 21st European Peptide Symposium (Giralt E., Andreu D., Eds.), ESCOM, Leiden, 1991, 849.

Butz S., Rawer S., Rapp W., Birsner U., Immunization and affinity purification of antibodies using resin-immobilized lysine-branched synthetic peptides, Pept. Res., 1994, 7 (1), 20-23.

Rapp W., PEG Grafted Polystyrene Tentacle Polymers in Combinatorial Peptide and Nonpeptide Libraries: A Handbook; Jung, G., Ed.; Wiley-VCH Verlag: Weinheim, Germany, 1996, 425.

Burkoth, T. S.; Benzinger, T. L. S.; Jones, D. N. M.; Hallenga, K.; Meredith, S. C.; Lynn, D. G. C-Terminal PEG Blocks the Irreversible Step in β-Amyloid(10-35) Fibrillogenesis. J. Am. Chem. Soc. 1998, 120 (30), 7655-7656. doi: 10.1021/ja980566b.