Swelling Volumes of Polystyrene, TentaGel® and HypoGel® Resins
| Water | MeOH | EtOH | DCM | Toluene | DMF | MeCN | THF | Dioxane | Ether | DMSO | |
| Polystyrene 1% DVB | -- |
1.6 | 1.7 | 8.3 | 8.5 | 5.6 | 3.2 | 8.8 | 7.8 | 4.0 | -- |
| Polystyrene 2% DVB | -- |
1.6 | 1.7 | 6 | 5.2 | 3.2 | 1.9 | 5.6 | 5.4 | -- | -- |
| TentaGel® S (0.25 - 0.3 mmol/g) | 3.6 | 3.6 | 2.9 | 6.3 | 4.8 | 4.7 | 4.2 | 5.0 | 5.4 | 1.9 | 3.9 |
| TentaGel® HL (0.4 - 0.6 mmol/g) | 3.1 | 3.6 | 3.5 | 5.7 | 4.1 | 4.6 | 3.9 | 4.2 | 4.8 | 2.4 | -- |
| TentaGel® macrobeads (0.25 - 0.35 mmol/g) | 4 | 5.2 | 3.9 | 4.9 | 6.2 | 6.6 | 5.9 | 6.5 | 6.9 | 2.1 | -- |
| HypoGel® 400 (0.6 - 0.8 mmol/g) | 1.8 | 2.9 | 2.8 | 6.9 | 5.5 | 5.2 | 4.6 | 5.3 | 5.6 | 2.6 | -- |
Table 1: Swelling volume [ml/g] of polystyrene (1% crosslinked, dry volume: 1.6 ml/g) and TentaGel® resins (dry volume 1.7 ml/g).
General Procedure for Measurement of Resin Swelling:
Literature: Synthesis of Peptides and Peptidomimetics, Houben Weyl, Eds. M. Goodman, A. Felix, L. Moroder, C. Toniolo, Georg Thieme Verlag Stuttgart, New York, (2002) Volume 22, p 676
To remove soluble by-products and impurities the resin has to be be washed carefully with toluene, DCM, DMF and methanol (5 times each solvent) and dried. To the dry resin (1 g in a 10-15 ml graduated cylinder) solvent is added and the resin is accurately mixed with the solvent (12-18 ml). The cylinder is sealed and after 2 h the resin is stirred again to remove all remaining air bubbles and to form a homogenous resin sus- pension. The cylinder is sealed again and after 22 h of settlement the resin volume is measured.