General Procedure for Measurement of Resin Swelling:

Literature: Synthesis of Peptides and Peptidomimetics, Houben Weyl, Eds. M. Goodman, A. Felix, L. Moroder, C. Toniolo, Georg Thieme Verlag Stuttgart, New York, (2002) Volume 22, p 676

To remove soluble by-products and impurities the resin has to be be washed carefully with toluene, CH2Cl2, DMF and methanol (5 times each solvent) and dried. To the dry resin (1 g in a 10-15 ml graduated cylinder) solvent is added and the resin is accurately mixed with the solvent (12-18 ml). The cylinder is sealed and after 2 h the resin is stirred again to remove all remaining air bubbles and to form a homogenous resin suspension. The cylinder is sealed again and after 22 h of settlement the resin volume is measured.